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Anti-GAD65 Antibody (C-Terminus) LS-C15432

Catalog Size Price
LS-C15432-500 500 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human GAD65
Human, Rat
IHC - Paraffin
Unconjugated

Details

Human GAD65
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

IHC - Paraffin

Specificity and Use

GAD65 antibody was raised against synthetic peptide derived from the C-terminus region of the human GAD 65 protein.
C-Terminus
Reacts with a 65kD protein, corresponding to human GAD (Glutamate Decarboxylase). Species cross-reactivity: Rat.
Suitable for use in Immunohistochemistry. Immunohistochemistry: 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive control: Pancreas.

Packaging

PBS, sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About GAD65

Q05329 NM_000818 NP_000809.1

GAD2 Antibody, GAD-65 Antibody, Glutamate decarboxylase 2 Antibody, GAD65 Antibody

GAD65 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.

Requested From: 
Date Requested: 6/29/2017

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