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Anti-GAD1 / GAD67 Antibody (aa526-537) IHC-plus™ LS-B3027

Note: This antibody replaces LS-C87394
Catalog Size Price
LS-B3027-50 50 µg (0.5 mg/ml) Unavailable

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100% Guaranteed
Goat Polyclonal to Human GAD1 / GAD67
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GAD1 / GAD67
Goat
Human, Mouse (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (4 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:8000)

Specificity and Use

GAD1 / GAD67 antibody was raised against synthetic peptide C-PDSPQRREKLHK from an internal region of human GAD1 / GAD67 (NP_000808.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (92%); Mouse, Rat, Elephant, Panda, Bat, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum (83%).
LS-E26954 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3027. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa526-537
Human GAD1 / GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Immunohistochemistry: LS-B3027 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3027 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kD according to Human NP_000808.2 and 66.6kD according to Mouse NP_032103.2). Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About GAD1 / GAD67

Q99259 NM_000817 NP_000808.2

GAD1 Antibody, CPSQ1 Antibody, GAD-67 Antibody, Glutamate decarboxylase 1 Antibody, GAD67 Antibody, GAD Antibody, SCP Antibody

GAD1 / GAD67 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.

Immunohistochemistry

Immunohistochemistry
Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 8/19/2017

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