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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAD1 / GAD67 Antibody (aa526‑537) IHC‑plus™ LS‑B3027
Note: This antibody replaces LS-C87394
GAD67 antibody LS-B3027 is an unconjugated goat polyclonal antibody to GAD67 (GAD1) from human and mouse. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3027-50
50 µg (0.5 mg/ml)
Unavailable

Popular GAD1 / GAD67 Products

Antibody
Species: Rat, Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-GAD67 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2028 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, ICC
Western blot Image
Species: Mouse, Rat
Applications: IHC, Western blot

Product Description

GAD67 antibody LS-B3027 is an unconjugated goat polyclonal antibody to GAD67 (GAD1) from human and mouse. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About GAD1 / GAD67
GAD1 / GAD67 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Q99259 NM_000817 NP_000808.2

GAD1 Antibody, CPSQ1 Antibody, GAD-67 Antibody, Glutamate decarboxylase 1 Antibody, GAD67 Antibody, GAD Antibody, SCP Antibody


Specifications

Target
Human GAD1 / GAD67
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GAD1 / GAD67 antibody was raised against synthetic peptide C-PDSPQRREKLHK from an internal region of human GAD1 / GAD67 (NP_000808.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (92%); Mouse, Rat, Elephant, Panda, Bat, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum (83%).
Blocking Peptide
LS-E26954 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3027. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa526-537
Specificity
Human GAD1 / GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Usage
Immunohistochemistry: LS-B3027 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3027 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kD according to Human NP_000808.2 and 66.6kD according to Mouse NP_032103.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.
Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.
Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.
Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.
Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3027 concentration 4 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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