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Anti-GABRA6 Antibody (Internal) IHC-plus™ LS-B3612

Note: This antibody replaces LS-C87390

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GABRA6 Antibodies

Anti-GABRA6 Antibody LS-C16
Rabbit Polyclonal to Rat GABRA6
Rat, Mouse
Western blot
Unconjugated
Western blot Image
Anti-GABRA6 Antibody (Internal) IHC-plus™ LS-B3612
Goat Polyclonal to Human GABRA6
Human, Monkey, Bovine, Dog, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GABRA6 Antibody (aa15-26) IHC-plus™ LS-B10335
Goat Polyclonal to Human GABRA6
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human GABRA6
Human, Monkey, Bovine, Dog, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GABRA6
Goat
Human, Monkey, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Horse, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (1:8000) & (1 - 3 µg/ml)

Specificity and Use

GABRA6 antibody was raised against peptide with sequence C-HPDSKYHLKKR, from the internal region of the protein sequence according to NP_000802.2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Pig (100%); Mouse, Rat, Rabbit, Horse (91%).
Internal
Human GABRA6.
Immunohistochemistry: LS-B3612 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3612 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 55kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 51.0kD according to NP_000802.2). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About GABRA6

Q16445 NM_000811 NP_000802.2

GABRA6 Antibody, GABA A receptor alpha 6 Antibody, GABA(A) receptor, alpha 6 Antibody

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor subtype. GABA-A receptors are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A receptor is a multimeric subunit complex.

Immunohistochemistry

Immunohistochemistry
Anti-GABRA6 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3612 concentration 2.5 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/5/2016

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