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Anti-GABARAP Antibody (aa1-30) LS-C166311


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GABARAP Antibodies

Anti-GABARAP Antibody (N-Terminus) IHC-plus™ LS-B283
Rabbit Polyclonal
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-GABARAP Antibody LS-C180114
Rabbit Polyclonal to Human GABARAP
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human GABARAP
IHC - Paraffin, Immunofluorescence, Western blot


Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

GABARAP antibody was raised against kLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human GABARAP.
LS-E5684 - Lyophilized - 100 µg - $145.00


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. American journal of hematology. 2013 [PubMed:23440701]


O95166 NM_007278 NP_009209.1

GABARAP Antibody, ATG8A Antibody, MM46 Antibody, FLC3B Antibody, GABARAP-a Antibody

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.


Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.


GABARAP antibody was tested in mouse MEFs and mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were anti-GABARAP (1:500, labeled with anti-rabbit 488 Alexa) and Covance anti-Tuj1 (1:1000, labeled with anti-mouse 647).


Fluorescent image of U251 cells stained with GABARAP antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated GABARAP primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot

Western blot
Rabbit anti-GABARAP 1:1000 dilution in 5% skimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film.

Western blot

Western blot
Western blot of GABARAP (arrow) using purified antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).

Requested From: United States
Date Requested: 10/27/2016

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