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Anti-G6PD Antibody (clone AT2F6) IHC-plus™ LS-B3587

Note: This antibody replaces LS-C103494
Catalog Size Price
LS-B3587-50 50 µl (1 mg/ml) Unavailable

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50 G6PD Antibodies

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Immunohistochemistry Image
Anti-G6PD Antibody (aa308-320) IHC-plus™ LS-B3253
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Anti-G6PD Antibody (clone AT2F6) IHC-plus™ LS-B3587
Mouse Monoclonal [clone AT2F6] (IgG2b,k) to Human G6PD
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone AT2F6] (IgG2b,k) to Human G6PD
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human G6PD
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT2F6]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

G6PD antibody was raised against recombinant human G6PD (35-506aa) purified from E. coli
human G6PD (35-506aa)
Immunohistochemistry: LS-B3587 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3587 was determined to be 5 ug/ml. Western Blot: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About G6PD

P11413 NM_000402 NP_000393.4

G6PD Antibody, G6PD1 Antibody

G6PD is glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms.

Immunohistochemistry

Immunohistochemistry
Anti-G6PD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3587 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-G6PD antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3587 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of MCF7 cell lysates.

Requested From: 
Date Requested: 8/21/2017

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