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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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G3BP1 / G3BP Antibody (aa401‑430) LS‑C166301
G3BP antibody LS-C166301 is an unconjugated rabbit polyclonal antibody to human G3BP (G3BP1). Validated for IHC and WB.
Catalog
Size
Price
LS-C166301-400
400 µl
Unavailable

Popular G3BP1 / G3BP Products

Anti-G3BP1 / G3BP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4491 concentration 5 ug/ml.
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Immunohistochemical analysis of G3BP1 (pS232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemical analysis of G3BP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Product Description

G3BP antibody LS-C166301 is an unconjugated rabbit polyclonal antibody to human G3BP (G3BP1). Validated for IHC and WB.
About G3BP1 / G3BP
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Q13283 NM_005754 NP_005745.1

G3BP1 Antibody, HDH-VIII Antibody, G3BP-1 Antibody, G3BP Antibody, GAP binding protein Antibody, HDH VIII Antibody


Specifications

Target
Human G3BP1 / G3BP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2706 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166301. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa401-430
Specificity
This G3BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human G3BP1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).
G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).

Immunohistochemistry

G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).
G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).

Immunohistochemistry

G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).
G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).

Immunohistochemistry

G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
G3BP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).
G3BP1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The G3BP1 antibody detected the G3BP1 protein (arrow).


Requested From: 
Date Requested: 6/20/2018

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