Human FZD2 / Frizzled 2

Goat

Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

aa267-279

Human FZD2 / Frizzled 2.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (10 µg/ml)
• Western blot (0.03 - 0.1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14332 NCBI: NM_001466 NP_001457.1