Human FXN / Frataxin

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Ascites

Unmodified

TrpE-Frataxin full length. Epitope: Maps to the first part of exon 4 of frataxin.

Recognizes only isoforms of human Frataxin containing exon 4. On Western Blots of normal human muscle, heart, cerebellum, and spinal cord extracts, antibody recognizes a band migrating at ~18kD corresponding to processed human frataxin (Campuzano, 1997). Slight cross reactivity with myosin may be observed by Western Blot. Immunofluorescent labeling of HeLa cells with This antibody demonstrates that frataxin is predominantly localized in the mitochondria (Campuzano, 1997). Species cross-reactivity: mouse.

• Immunofluorescence (1:50 - 1:500)
• Western blot
• ELISA

Suitable for use in Western Blot, Immunofluorescence and ELISA. Western Blot (Natural and recombinant protein): 1:2500. Immunofluorescence: 1:50-1:500. Fixation of cells in ice cold acetone or 4% paraformaldehyde is recommended. Due to subcellular localization of frataxin in the mitochondria, cells should be permeabilized in the presence of detergent prior to incubation with primary antibody. Positive control: Liver, heart or skeletal muscle.

PBS, 40% Glycerol, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16595 NCBI: NM_000144 NP_000135.2