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Anti-FST / Follistatin Antibody (aa30-329) LS-C149948

Catalog Size Price
LS-C149948-25 25 µg Unavailable
LS-C149948-100 100 µg Unavailable

Most Popular FST / Follistatin Antibodies

Anti-FST / Follistatin Antibody (C-Terminus) LS-C31897
Rabbit Polyclonal (IgG) to Human FST / Follistatin
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep
Western blot
Unconjugated
Western blot Image
Anti-FST / Follistatin Antibody (Biotin) LS-C104465
Rabbit Polyclonal to Human FST / Follistatin
Human
Western blot, ELISA
Biotin Conjugated
Anti-FST / Follistatin Antibody IHC-plus™ LS-B7434
Rabbit Polyclonal (IgG) to Human FST / Follistatin
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-FST / Follistatin Antibody (aa312-340) IHC-plus™ LS-B10674
Rabbit Polyclonal to Human FST / Follistatin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FST / Follistatin Antibody (HRP) LS-C546271
Rabbit Polyclonal (IgG) to Human FST / Follistatin
Human
Western blot, ELISA
HRP Conjugated

100% Guaranteed
Goat Polyclonal (IgG) to Human FST / Follistatin
Human
IHC - Paraffin, Western blot, Neutralization
Unconjugated

Details

Human FST / Follistatin
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Neutralization

Specificity and Use

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Follistatin Gly30-Asp329 Accession # P19883.
aa30-329
Detects human Follistatin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse.
Western Blot, 0.1 µg/ml, Recombinant Human Follistatin 300 aa 30-329. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human placenta. Neutralization, Measured by its ability to neutralize Follistatin inhibition of Activin A-dependent hemoglobin expression in the K562. human chronic myelogenous leukemia cell line. At 6 µg/ml, this antibody will neutralize approximately 60-85% of rhFollistatin 300 bioactivity on K562 cells.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About FST / Follistatin

P19883 NM_006350 NP_006341.1

FST Antibody, Activin-binding protein Antibody, Follistatin isoform FST317 Antibody, Follistatin Antibody, Fs Antibody

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.

Neutralization

Neutralization
Follistatin Inhibition of Activin A-induced Hemoglobin Expression and Neutralization by Human Follistatin Antibody. Recombinant Human Follistatin 300 inhibits Recombinant Human/Mouse/Rat Activin A induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/ml) activity elicited by Recombinant Human Follistatin 300 (0.4 ug/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin Antigen Affinity-purified Polyclonal Antibody. At 6 ug/m L, this antibody will neutralize approximately 60-85% of rhFollistatin 300 bioactivity.

Requested From: 
Date Requested: 12/16/2017

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