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Antibodies > FSH > LS-C355416
 
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Catalog Number Size Price
LS-C355416-1 1 mg $857 
FSH Antibody - Immunofluorescence analysis of Follicle Stimulating Hormone was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Follicle Stimulating Hormone (P2B4) Mouse Monoclonal Antibody at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
FSH Antibody - Western blot analysis was performed on 5 ul of Normal Female Human Plasma (Lane 1) and tissue extract (30 µg lysate) of Mouse ovary (Lane 2). The blot was probed with Anti-Follicle Stimulating Hormone Mouse monoclonal and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~21 kDa band corresponding to Follicle Stimulating Hormone was observed across the plasma and tissue tested. Known quantity of protein samples were electrophoresed using 10 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody.
FSH Antibody - Immunofluorescence analysis of Follicle Stimulating Hormone was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Follicle Stimulating Hormone (P2B4) Mouse Monoclonal Antibody at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
FSH Antibody - Western blot analysis was performed on 5 ul of Normal Female Human Plasma (Lane 1) and tissue extract (30 µg lysate) of Mouse ovary (Lane 2). The blot was probed with Anti-Follicle Stimulating Hormone Mouse monoclonal and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~21 kDa band corresponding to Follicle Stimulating Hormone was observed across the plasma and tissue tested. Known quantity of protein samples were electrophoresed using 10 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody.
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Monoclonal Mouse anti‑Human FSH Antibody (clone P2B4, IF, WB) LS‑C355416

Monoclonal Mouse anti‑Human FSH Antibody (clone P2B4, IF, WB) LS‑C355416

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Antibody:
FSH Mouse anti-Human Monoclonal (P2B4) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355416-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
FSH Mouse anti-Human Monoclonal (P2B4) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FSH antibody LS-C355416 is an unconjugated mouse monoclonal antibody to human FSH. Validated for ELISA, IF, IP, RIA and WB.
Target
Human FSH
Synonyms
FSH
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
P2B4
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Human Follicle Stimulating Hormone
Specificity
The antibody targets Follicle Stimulating Hormone in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Follicle Stimulating Hormone which has a predicted molecular weight of approximately 13 kD.
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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