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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FREM1 Antibody (C‑Terminus) IHC‑plus™ LS‑B5486 Best Seller
Note: This antibody replaces LS-C115864
FREM1 antibody LS-B5486 is an unconjugated rabbit polyclonal antibody to FREM1 from human and mouse. Validated for ELISA, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5486-50
50 µg (1 mg/ml)
Unavailable

Popular FREM1 Products

Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, ELISA
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: ELISA

Product Description

FREM1 antibody LS-B5486 is an unconjugated rabbit polyclonal antibody to FREM1 from human and mouse. Validated for ELISA, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About FREM1
FREM1 is a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. Q5H8C1 NP_659403.4

FREM1 Antibody, BNAR Antibody, C9orf143 Antibody, C9orf145 Antibody, C9orf154 Antibody, TRIGNO2 Antibody, TILRR Antibody, MOTA Antibody, Protein QBRICK Antibody


Specifications

Target
Human FREM1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30956 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B5486. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5-1 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.
Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.

Immunohistochemistry

Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.
Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.

Immunohistochemistry

Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.
Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.

Immunohistochemistry

Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.
Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5486 concentration 10 ug/ml.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunocytochemistry

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1µg/mL.

Western blot

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/ml.


Requested From: 
Date Requested: 6/19/2018

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