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Anti-Fragilis / IFITM3 Antibody (aa1-30) LS-C98676

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Fragilis / IFITM3 Antibodies

Anti-Fragilis / IFITM3 Antibody (aa64-93) LS-C98677
Rabbit Polyclonal to Human Fragilis / IFITM3
Human
Western blot
Unconjugated
Western blot Image
Anti-Fragilis / IFITM3 Antibody (Internal, clone EPR5242) IHC-plus™ LS-B7510
Rabbit Monoclonal [clone EPR5242] to Human Fragilis / IFITM3
Human
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human Fragilis / IFITM3
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human Fragilis / IFITM3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • ICC
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000)

Specificity and Use

Fragilis / IFITM3 antibody was raised against kLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human Interferon-inducible protein (IFITM3).
LS-E1055 - Lyophilized - 100 µg - $145.00
aa1-30

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
IFITM3 inhibits influenza A virus infection by preventing cytosolic entry. Feeley EM, Sims JS, John SP, Chin CR, Pertel T, Chen LM, Gaiha GD, Ryan BJ, Donis RO, Elledge SJ, Brass AL. PLoS pathogens. 2011 7:e1002337. [PubMed:22046135] [PMC:PMC3203188]

About Fragilis / IFITM3

Q01628 NM_021034 NP_066362.2

IFITM3 Antibody, 1-8U Antibody, Dispanin subfamily A member 2b Antibody, DSPA2b Antibody, Fragilis Antibody, IP15 Antibody

IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cells stained with IFITM3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.

Western blot

Western blot
Western blot of lysate from HT1080 cell line,using IFITM3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

Western blot

Western blot
IFITM3 Antibody western blot of HeLa,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35 ug/lane). The IFITM3 antibody detected the IFITM3 protein (arrow).
 

Requested From: United States
Date Requested: 12/9/2016

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