Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100)
ICC (1:400 - 1:1000)
Western blot (1:1000 - 1:4000)
Specificity and Use
Fractin antibody was raised against k-YELPD peptide representing the last five amino acids of the C-terminus of the 32kD Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig.
By immunohistochemistry and Western blot, this antibody recognizes the 32kD Fractin fragment but not full-length beta actin.
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Immunohistochemistry: 1:100. If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended. Western Blot: 1:1000-1:4000 for incubation overnight at 4?C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4. Positive control: Staurosporin treated neuronal cultures.
Whole rabbit serum. No preservative added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
High sphingomyelin levels induce lysosomal damage and autophagy dysfunction in Niemann Pick disease type A. Gaband[Character e9]-Rodr[Character ed]guez E, Boya P, Labrador V, Dotti CG, Ledesma MD. Cell death and differentiation. 2014 Jun;21:864-75. [Full Text Article]