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Anti-FOXP3 Antibody (aa105-200) LS-C149953


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular FOXP3 Antibodies

Anti-FOXP3 Antibody (aa100-149) LS-C31026
Rabbit Polyclonal to Human FOXP3
Human, Monkey, Rat, Bovine, Pig, Sheep
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-FOXP3 Antibody LS-C312052
Rabbit Polyclonal (IgG) to Human FOXP3
IHC - Paraffin, IHC - Frozen
Immunohistochemistry Image
Anti-FOXP3 Antibody (aa101-431) LS-C357445
Rabbit Polyclonal (IgG) to Human FOXP3
Human, Mouse, Rat
IHC - Paraffin
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human FOXP3
IHC - Paraffin, ICC, Western blot, Immunoprecipitation


Human FOXP3
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin
  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation

Specificity and Use

FOXP3 antibody was raised against e. coli-derived recombinant human FoxP3 Gln105-Lys200 Accession # Q9-BZS1.
Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is.
Western Blot, 1 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 5-15 µg/ml, Immersion fixed human peripheral blood mononuclear cells. Immunohistochemistry, 5-15 µg/ml.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About FOXP3

Q9BZS1 NM_014009 NP_054728.2

FOXP3 Antibody, AIID Antibody, DIETER Antibody, Forkhead box protein P3 Antibody, JM2 Antibody, Forkhead box P3 Antibody, FOXP3delta7 Antibody, XPID Antibody, PIDX Antibody, SCURFIN Antibody

FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.


FoxP3 in Human Tonsil. FoxP3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 10 ug/ml Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human FoxP3 by Western Blot. Western blot shows lysates of human CD4+CD25+ T cells and human CD4+CD25T cells. PVDF membrane was probed with 1 ug/ml of Goat Anti Human FoxP3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti Goat IgG Secondary Antibody. A specific band was detected for FoxP3 at approximately 47 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of FoxP3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. FoxP3/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human FoxP3 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The IL-2 promoter was detected by standard PCR.

Requested From: United States
Date Requested: 10/23/2016

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