Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
FOXP1 belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains.
FoxP1 in MCF-7 Human Cell Line. FoxP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human FoxP1 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 ug of whole cell lysate (WCL), 20 ug of cytoplasmic (Cyto), and 10 ug of nuclear extracts (Nuc). PVDF membrane was probed with 1 ug/ml Mouse Anti-Human FoxP1 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific bands for FoxP1 were detected at approximately 60, 85, and 100 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 2/27/2017