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Anti-FOXO3 / FOXO3A Antibody (phospho-Ser253) LS-C123585

Catalog Size Price
LS-C123585-50 50 µg (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human FOXO3 / FOXO3A
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated

Details

Human FOXO3 / FOXO3A
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:4000)

Specificity and Use

FOXO3 / FOXO3A antibody was raised against synthetic phosphopeptide corresponding to human FOXO3A around the phosphorylation site of serine 253 (A-V-SP-M-D).
pSer253
Recognizes human FOXO3A when phosphorylated at serine 253. Species cross-Reactivity: mouse, rat.
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:4000. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.

About FOXO3 / FOXO3A

O43524 NM_001455 NP_001446.1

FOXO3 Antibody, AF6q21 Antibody, AF6q21 protein Antibody, Forkhead box O3 Antibody, Forkhead box O3A Antibody, FOXO3A Antibody, FKHRL1P2 Antibody, Forkhead box protein O3 Antibody, FOXO2 Antibody, FKHRL1 Antibody

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Requested From: 
Date Requested: 5/25/2018

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