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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXH1 Antibody (N‑Terminus) LS‑C116166
FOXH1 antibody LS-C116166 is an unconjugated rabbit polyclonal antibody to FOXH1 from human, bat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C116166-50
50 µg
Unavailable

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Product Description

FOXH1 antibody LS-C116166 is an unconjugated rabbit polyclonal antibody to FOXH1 from human, bat, bovine and other species. Validated for WB.
About FOXH1
Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). O75593 NM_003923 NP_003914.1

FOXH1 Antibody, Fast-2 Antibody, FAST2 Antibody, FAST-1 Antibody, FAST1 Antibody, Forkhead box H1 Antibody, Forkhead box protein H1 Antibody, HFAST-1 Antibody


Specifications

Target
Human FOXH1
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Dog, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
FOXH1 antibody was raised against synthetic peptide from N-Terminus of human FOXH1 (O75593, NP_003914). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%); Galago, Horse (92%); Panda, Dog (91%); Elephant, Bovine (85%); Pig (83%).
Blocking Peptide
LS-E13798 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116166. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human FOXH1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXH1 antibody LS-C116166 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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