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FOXC1 Antibody (aa541‑553) IHC‑plus™ LS‑B2996
Note: This antibody replaces LS-C54851, LS-C66329
FOXC1 antibody LS-B2996 is an unconjugated goat polyclonal antibody to FOXC1 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Monkey, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Rat, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
FOXC1 antibody LS-B2996 is an unconjugated goat polyclonal antibody to FOXC1 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About FOXC1
Forkhead box C1 (FOXC1) belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of FOXC1 has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in FOXC1 cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.
Q12948
NM_001453
NP_001444.2
FOXC1 Antibody, Forkhead-like 7 Antibody, FREAC3 Antibody, FKHL7 Antibody, Forkhead-related activator 3 Antibody, Forkhead-related protein FKHL7 Antibody, IGDA Antibody, IHG1 Antibody, IRID1 Antibody, Forkhead box protein C1 Antibody, Mesenchyme fork head protein 1 Antibody, Myeloid factor-delta Antibody, ARA Antibody, Forkhead box C1 Antibody, FREAC-3 Antibody, RIEG3 Antibody
Specifications
Target
Human FOXC1
Host
Goat
Reactivity
Human, Monkey, Mouse, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.5 - 1.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Immunogen
FOXC1 antibody was raised against synthetic peptide RTSGAFVYDCSKF from the C-terminus of human FOXC1 (NP_001444.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Elephant, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (100%); Salmon (92%).
Blocking Peptide
LS-E25561 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2996. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-B2996. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa541-553
Specificity
Human FOXC1.
Usage
Immunohistochemistry: LS-B2996 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2996 was determined to be 3.75 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Western blot
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Western blot
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Western blot
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Immunohistochemistry
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Western blot
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Requested From:
Date Requested: 6/22/2018
Date Requested: 6/22/2018
