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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXC1 Antibody (aa541‑553) IHC‑plus™ LS‑B2996
Note: This antibody replaces LS-C54851, LS-C66329
FOXC1 antibody LS-B2996 is an unconjugated goat polyclonal antibody to FOXC1 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2996-50
50 µg (0.5 mg/ml)
Unavailable

Popular FOXC1 Products

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1800 dilution 1:200.
Species: Human, Monkey, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4579 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Western blot of recombinant FOXC1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Immunofluorescent analysis of Hela cells using CSB-PA618640LA01HU at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

FOXC1 antibody LS-B2996 is an unconjugated goat polyclonal antibody to FOXC1 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About FOXC1
Forkhead box C1 (FOXC1) belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of FOXC1 has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in FOXC1 cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. Q12948 NM_001453 NP_001444.2

FOXC1 Antibody, Forkhead-like 7 Antibody, FREAC3 Antibody, FKHL7 Antibody, Forkhead-related activator 3 Antibody, Forkhead-related protein FKHL7 Antibody, IGDA Antibody, IHG1 Antibody, IRID1 Antibody, Forkhead box protein C1 Antibody, Mesenchyme fork head protein 1 Antibody, Myeloid factor-delta Antibody, ARA Antibody, Forkhead box C1 Antibody, FREAC-3 Antibody, RIEG3 Antibody


Specifications

Target
Human FOXC1
Host
Goat
Reactivity
Human, Monkey, Mouse, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.5 - 1.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXC1 antibody was raised against synthetic peptide RTSGAFVYDCSKF from the C-terminus of human FOXC1 (NP_001444.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Elephant, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (100%); Salmon (92%).
Blocking Peptide
LS-E25561 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2996. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa541-553
Specificity
Human FOXC1.
Usage
Immunohistochemistry: LS-B2996 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2996 was determined to be 3.75 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Western blot

Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Western blot

Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Western blot

Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2996 concentration 75 ug/ml.

Western blot

Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/22/2018

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