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Anti-FOS / c-FOS Antibody (aa75-155) LS-C6879

Ordering

Wt. Vol. Conc. Price
100 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FOS / c-FOS Antibodies

Anti-FOS / c-FOS Antibody (aa4-17) LS-C4270
Sheep Polyclonal (IgG) to Human FOS / c-FOS
Human, Rat
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
Anti-FOS / c-FOS Antibody (aa128-152, clone 2G9C3) LS-C77393
Mouse Monoclonal [clone 2G9C3] (IgG2a) to Human FOS / c-FOS
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken
Immunofluorescence, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human FOS / c-FOS
Human, Mouse
ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human FOS / c-FOS
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 10 µg/ml)
  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation

Specificity and Use

FOS / c-FOS antibody was raised against trp E fusion-protein corresponding to the N-terminal fragment containing aa75-155 of human v-Fos.
aa75-155
Recognizes human c-Fos and v-Fos, 55kD. An additional band may be detected at 116kD. Species cross-reactivity: mouse.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4-10 ug immunoprecipitated Fos from 100 ug of a RIPA lysate of human HeLa cells. Immunocytochemistry: 5-10 ug/ml has produced positive immunostaining of HeLa cells that had been serum-starved and then serum-stimulated for 30 minutes prior to fixation with 4% paraformaldehyde followed by 1% Triton-X. Positive control: H1838: HeLa Nuclear Extract.

Packaging

PBS, 2 mg/ml BSA, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About FOS / c-FOS

P01100 NM_005252 NP_005243.1

FOS Antibody, Activator protein 1 Antibody, AP-1 Antibody, Cellular oncogene c-fos Antibody, Cellular oncogene fos Antibody, Proto-oncogene c-Fos Antibody, v-fos Antibody, C-FOS Antibody, G0s7 Antibody, p55 Antibody

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton.

Requested From: United States
Date Requested: 12/3/2016

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