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Anti-FOLH1 / PSMA Antibody (aa117-351, clone K1H7) IHC-plus™ LS-B2950

Note: This antibody replaces LS-C93789

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FOLH1 / PSMA Antibodies

Anti-FOLH1 / PSMA Antibody (clone 3E6) LS-C95615
Mouse Monoclonal [clone 3E6] (IgG1) to Human FOLH1 / PSMA
Human
IHC
Unconjugated
Anti-FOLH1 / PSMA Antibody (clone 107.1A4) LS-C150527
Mouse Monoclonal [clone 107.1A4] (IgG1)
Human
IHC - Paraffin, IHC - Frozen, ICC, Flow Cytometry, ELISA
Unconjugated
Immunocytochemistry Image
Anti-FOLH1 / PSMA Antibody (aa161-190) LS-C166202
Rabbit Polyclonal to Human FOLH1 / PSMA
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone K1H7] (IgG1,k) to Human FOLH1 / PSMA
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FOLH1 / PSMA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [K1H7]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:3000)
  • ELISA

Specificity and Use

FOLH1 / PSMA antibody was raised against recombinant human PSMA (117-351aa) purified from E. coli.
aa117-351
Immunohistochemistry: LS-B2950 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2950 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About FOLH1 / PSMA

Q04609 NM_004476 NP_004467.1

FOLH1 Antibody, Folate hydrolase 1 Antibody, GCPII Antibody, Glutamate carboxypeptidase II Antibody, Folate hydrolase Antibody, GCP2 Antibody, NAALAD1 Antibody, NAALAdase Antibody, NAALADase I Antibody, PSM Antibody, FGCP Antibody, FOLH Antibody, Glutamate carboxylase II Antibody, Glutamate carboxypeptidase 2 Antibody, MGCP Antibody, PSMA Antibody

FOLH1 / PSMA is a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney.

Immunohistochemistry

Immunohistochemistry
Anti-FOLH1 / PSMA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2950 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FOLH1 / PSMA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2950 concentration 5 ug/ml.

Western blot

Western blot
Cell lysates of LNCaP(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/5/2016

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