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Antibodies > FN1 / Fibronectin > LS-C355423
 
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Catalog Number Size Price
LS-C355423-1 1 mg $857 
FN1 / Fibronectin Antibody - Immunofluorescent analysis of Fibronectin (green) in HepG2 cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum for 15 minutes at room temperature. Cells were stained with or without Fibronectin monoclonal antibody, at a dilution of 1:100 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
FN1 / Fibronectin Antibody - WB using Fibronectin Antibody (3F12)
FN1 / Fibronectin Antibody - Sandwich ELISA of Fibronectin was performed by coating wells of a 96-well plate with 100ul of an fibronectin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of a fibronectin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
FN1 / Fibronectin Antibody - Direct ELISA analysis of Ferritin was performed by coating wells of a 96-well plate with 100ul per well of Human Ferritin or BSA diluted in carbonate/bicarbonate buffer, starting at a concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 2ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-ferritin monoclonal antibody at a concentration of 1 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:25000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.
FN1 / Fibronectin Antibody - Immunofluorescent analysis of Fibronectin (green) in HepG2 cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum for 15 minutes at room temperature. Cells were stained with or without Fibronectin monoclonal antibody, at a dilution of 1:100 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
FN1 / Fibronectin Antibody - WB using Fibronectin Antibody (3F12)
FN1 / Fibronectin Antibody - Sandwich ELISA of Fibronectin was performed by coating wells of a 96-well plate with 100ul of an fibronectin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of a fibronectin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
FN1 / Fibronectin Antibody - Direct ELISA analysis of Ferritin was performed by coating wells of a 96-well plate with 100ul per well of Human Ferritin or BSA diluted in carbonate/bicarbonate buffer, starting at a concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 2ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-ferritin monoclonal antibody at a concentration of 1 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:25000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.
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Monoclonal Mouse anti‑Human FN1 / Fibronectin Antibody (clone 3F12, IF, WB) LS‑C355423

Monoclonal Mouse anti‑Human FN1 / Fibronectin Antibody (clone 3F12, IF, WB) LS‑C355423

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Antibody:
FN1 / Fibronectin Mouse anti-Human Monoclonal (3F12) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355423-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
FN1 / Fibronectin Mouse anti-Human Monoclonal (3F12) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Fibronectin antibody LS-C355423 is an unconjugated mouse monoclonal antibody to human Fibronectin (FN1). Validated for ELISA, IF, IP, RIA and WB.
Target
Human FN1 / Fibronectin
Synonyms
FN1 | CIG | Cold-insoluble globulin | Fibronectin 1 | FINC | LETS | GFND | ED-B | Fibronectin | FN | FNZ | GFND2 | Migration-stimulating factor | MSF
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
3F12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Human plasma fibronectin
Specificity
The antibody targets Fibronectin in ELISA, WB, IP, and RIA applications and shows reactivity with Human samples. It detects Fibronectin which has a predicted molecular weight of approximately 259 kD.
Applications
  • Immunofluorescence
  • Western blot (1:100 - 1:500)
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FN1 / Fibronectin
P02751 NM_002026 NP_002017.1

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FN1 / Fibronectin Antibody - Anti-FN1 / Fibronectin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.
IHC‑plus™ FN1 / Fibronectin Antibody LS‑B382
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FN1 / Fibronectin Antibody (APC) LS‑C129078
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FN1 / Fibronectin Antibody (Biotin) LS‑C23632
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
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FN1 / Fibronectin Antibody (HRP) LS‑C212043
Species: Human
Applications: ELISA
FN1 / Fibronectin Antibody - Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2 ug/ml for 1 hour @ room T. Secondary antibody: Streptavidin. Conj. HRP 10 ug/ml circa 45 min. @ room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FN1 / Fibronectin Antibody (Biotin) LS‑C60153
Species: Human, Monkey, Mouse, Rat, Bovine
Applications: IHC, Western blot, Immunoprecipitation, ELISA
See all FN1 / Fibronectin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/22/2024
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