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Anti-FN1 / Fibronectin Antibody (Biotin) LS-C60153


Wt. Vol. Conc. Price
100 µg 0.1 ml - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular FN1 / Fibronectin Antibodies

Anti-FN1 / Fibronectin Antibody IHC-plus™ LS-B382
Rabbit Polyclonal to Human FN1 / Fibronectin
IHC - Paraffin, ELISA
Immunohistochemistry Image
Anti-FN1 / Fibronectin Antibody (FITC) IHC-plus™ LS-B7943
Sheep Polyclonal (IgG) to Human FN1 / Fibronectin
Human, Dog
IHC - Paraffin, Immunofluorescence, ELISA
FITC Conjugated
Immunohistochemistry Image
Anti-FN1 / Fibronectin Antibody IHC-plus™ LS-B13204
Rabbit Polyclonal (IgG) to Human FN1 / Fibronectin
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human FN1 / Fibronectin
Human, Monkey, Mouse, Rat, Bovine
IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Biotin Conjugated


Human FN1 / Fibronectin
Human, Monkey, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Frozen (1:50 - 1:200)
  • Western blot (1:10000)
  • Immunoprecipitation
  • ELISA (1:4000 - 1:8000)

Specificity and Use

FN1 / Fibronectin antibody was raised against fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single- band purity was assessed by SDS-PAGE.
other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross- reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible
Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.


Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
0.1 ml deionized water
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only.

About FN1 / Fibronectin

P02751 NM_002026 NP_002017.1

FN1 Antibody, CIG Antibody, Cold-insoluble globulin Antibody, Fibronectin 1 Antibody, FINC Antibody, LETS Antibody, GFND Antibody, ED-B Antibody, Fibronectin Antibody, FN Antibody, FNZ Antibody, GFND2 Antibody, Migration-stimulating factor Antibody, MSF Antibody

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.


Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2 ug/ml for 1 hour @ room T. Secondary antibody: Streptavidin. Conj. HRP 10 ug/ml circa 45 min. @ room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Requested From: United States
Date Requested: 10/23/2016

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