Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Fluorescein antibody was raised against fluorescein conjugated to Goat IgG.
Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.
Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:1000 to 1:5000, 1.0 ug of Fluorescein conjugated IgG in a standard capture using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50% glycerol;, pH 8.0, 10 mg/ml BSA, (IgG, protease free), 0.01% sodium azide.