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Catalog Number Size Price
LS-B9180-50 50 µg (0.5 mg/ml) $485 
FLOT1 / Flotillin 1 Antibody - Anti-FLOT1 / Flotillin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FLOT1 / Flotillin 1 Antibody - FLOT1 antibody staining (0.3 ug/ml) of H460 lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
FLOT1 / Flotillin 1 Antibody - Anti-FLOT1 / Flotillin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FLOT1 / Flotillin 1 Antibody - FLOT1 antibody staining (0.3 ug/ml) of H460 lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human FLOT1 / Flotillin 1 Antibody (aa415‑427, IHC, WB) LS‑B9180

IHC‑plus™ Polyclonal Goat anti‑Human FLOT1 / Flotillin 1 Antibody (aa415‑427, IHC, WB) LS‑B9180

Note: This antibody replaces LS-C34645
Antibody:
FLOT1 / Flotillin 1 Goat anti-Human Polyclonal (aa415-427) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B9180-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FLOT1 / Flotillin 1 Goat anti-Human Polyclonal (aa415-427) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Flotillin 1 antibody LS-B9180 is an unconjugated goat polyclonal antibody to Flotillin 1 (FLOT1) (aa415-427) from human. It is reactive with human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FLOT1 / Flotillin 1
Synonyms
FLOT1 | Flotillin-1 | Flotillin 1
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-SISQVNHKPLRTA, from the C-Terminus of protein sequence according to NP_005794.1NP_001305804.1.
Epitope
aa415-427
Specificity
Human FLOT1 / Flotillin 1.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell line H460 (calculated MW of 47.4 kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FLOT1 / Flotillin 1
O75955 NM_005803 NP_005794.1

Validation

FLOT1 / Flotillin 1 Antibody - Anti-FLOT1 / Flotillin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLOT1 / Flotillin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLOT1 / Flotillin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ FLOT1 / Flotillin 1 Antibody (aa415-427) for IHC, WB/Western LS-B9180 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024