Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Feline Immunodeficiency Virus, Cat (tested or 100% immunogen sequence identity)
Broad (at least 90% immunogen sequence identity)
IgG1 Monoclonal [SU1-30]
Specificity and Use
Recognizes the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU, gp95. The primary receptor for FIV infection of cats has been identified as the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion in the CD4+ population. Studies have shown that the V3 loop of SU, gp95 is a ma a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU, gp95 to CXCR4. Clone SU1-30 has been shown to inhibit the sCD134-activated entry of both clade B and clade C FIV strains. Does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of Sodium Azide is recommended prior to use in functional studies.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.