FITC

Fluorescein Isothiocyanate (FITC)

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.

FITC Target Details

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Products

Antibodies (107)

Type

Primary (107)

Target

FITC (107)

Reactivity

Human (9)

Mouse (3)

All species (8)

Synthetic (8)

Application

IHC (18)

IHC-Fr (7)

IHC-P (1)

WB (46)

Flo (24)

ELISA (74)

FLISA (1)

ICC (9)

IF (6)

IP (17)

RIA (2)

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Host

rabbit (20)

mouse (54)

goat (1)

hamster-armenian (1)

human (5)

rat (9)

sheep (17)

Isotype

Fab (2)

IgG (30)

IgG,k (2)

IgG1 (18)

IgG1,k (7)

IgG2,k (1)

IgG2a (13)

IgG2a,k (7)

IgG2b,k (10)

IgG2c,k (2)

IgG3,k (2)

IgG4,k (1)

IgM (5)

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Clonality

monoclonal mc (53)

polyclonal pc (37)

recombinant monoclonal rmc (17)

Clone

#8 (1)

#9 (1)

2A3 (1)

4-4-20 (enhanced) (17)

F4/1 (1)

LO-FLUO-1 (2)

NI 239 (8)

Format

AP Conjugated (2)

APC (1)

APC Conjugated (7)

Biotin Conjugated (17)

Cy3 Conjugated (1)

Cy7 Conjugated (1)

FITC Conjugated (5)

HRP Conjugated (14)

PE Conjugated (12)

Sepharose Conjugated (1)

Unconjugated (47)

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Epitope

Fluorescein (17)

N-Terminus (1)

Publications

No (107)

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FITC Antibody (clone F4/1) LS-C34608

Antibody:

FITC Mouse Monoclonal (F4/1) Antibody

Applications:

Flo, IHC, IHC-Fr, IP

Format:

Unconjugated

Price:

250 µg/$436

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FITC Antibody (N-Terminus) LS-C56216

Antibody:

FITC Rabbit anti-Synthetic Polyclonal (N-Terminus) Antibody

Reactivity:

Synthetic

Applications:

ELISA, IHC-Fr

Format:

Unconjugated

Price:

1 mg/$536

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FITC Antibody LS-C212393

Antibody:

FITC Mouse Monoclonal Antibody

Applications:

ELISA, WB

Format:

Unconjugated

Price:

50 µg/$316; 100 µg/$386

Select

FITC Antibody LS-C66236

Antibody:

FITC Sheep Polyclonal Antibody

Applications:

ELISA

Format:

Unconjugated

Price:

1 mg/$520

Select

FITC Antibody LS-C835472

Antibody:

FITC Rabbit Polyclonal Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

Unconjugated

Price:

1 ml/$388

Select

FITC Antibody (clone LO-FLUO-1) LS-C57296

Antibody:

FITC Rat anti-Synthetic Monoclonal (LO-FLUO-1) Antibody

Reactivity:

Synthetic

Applications:

ELISA, Flo

Format:

Unconjugated

Price:

0.5 mg/$525

Select

FITC Antibody (clone NI 239) LS-C147255

Antibody:

FITC Mouse Monoclonal (NI 239) Antibody

Applications:

ELISA, WB

Format:

Unconjugated

Price:

0.2 mg/$481

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FITC Antibody (clone LO-FLUO-1, Biotin) LS-C57297

Antibody:

FITC Rat anti-Synthetic Monoclonal (Biotin) (LO-FLUO-1) Antibody

Reactivity:

Synthetic

Applications:

ELISA, Flo

Format:

Biotin Conjugated

Price:

0.5 mg/$561

Select

FITC Antibody (HRP) LS-C27211

Antibody:

FITC Rabbit anti-Synthetic Polyclonal (HRP) Antibody

Reactivity:

Synthetic

Applications:

ELISA, IHC-Fr, WB

Format:

HRP Conjugated

Price:

1 ml/$545

Select

FITC Antibody (APC) LS-C516817

Antibody:

FITC Rabbit Polyclonal (APC) Antibody

Applications:

ELISA, IP, WB

Format:

APC Conjugated

Price:

100 µl/$832

Select

FITC Antibody LS-C56403

Antibody:

FITC Sheep anti-Synthetic Polyclonal Antibody

Reactivity:

Synthetic

Applications:

ELISA, IHC-Fr

Format:

Unconjugated

Price:

1 mg/$525

Select

FITC Antibody (Biotin) LS-C56406

Antibody:

FITC Sheep anti-Synthetic Polyclonal (Biotin) Antibody

Reactivity:

Synthetic

Applications:

ELISA, IHC-Fr

Format:

Biotin Conjugated

Price:

1 mg/$552

Select

FITC Antibody (Biotin) LS-C66239

Antibody:

FITC Sheep Polyclonal (Biotin) Antibody

Applications:

ELISA

Format:

Biotin Conjugated

Price:

1 mg/$471

Select

FITC Antibody (PE) LS-C66240

Antibody:

FITC Sheep Polyclonal (PE) Antibody

Applications:

ELISA, IF, IHC, WB

Format:

PE Conjugated

Price:

500 µg/$533

Select

FITC Antibody (AP) LS-C66237

Antibody:

FITC Sheep Polyclonal (AP) Antibody

Applications:

ELISA, IHC, WB

Format:

AP Conjugated

Price:

1 ml/$459

• Biotin
• PE
• AP

• Biotin
• PE
• AP

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FITC Antibody (HRP) LS-C351760

Antibody:

FITC Sheep Polyclonal (HRP) Antibody

Applications:

ELISA

Format:

HRP Conjugated

Price:

1 ml/$319

Select

FITC Antibody LS-C835467

Antibody:

FITC Goat Polyclonal Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

Unconjugated

Price:

1 ml/$350

Select

FITC Antibody (clone #8) LS-C355952

Antibody:

FITC Mouse Monoclonal (#8) Antibody

Applications:

ELISA, IP, RIA, WB

Format:

Unconjugated

Price:

1 mg/$857

Select

FITC Antibody (clone #9) LS-C355956

Antibody:

FITC Mouse Monoclonal (#9) Antibody

Applications:

ELISA, IP, RIA, WB

Format:

Unconjugated

Price:

1 mg/$857

Select

FITC Antibody LS-C66249

Antibody:

FITC Mouse Monoclonal Antibody

Applications:

ELISA, IHC

Format:

Unconjugated

Price:

1 mg/$786

Select

FITC Antibody (HRP) LS-C546222

Antibody:

FITC Mouse Monoclonal (HRP) Antibody

Applications:

ELISA, IF, IHC

Format:

HRP Conjugated

Price:

100 µl/$962

Select

FITC Antibody (Biotin) LS-C526109

Antibody:

FITC Mouse Monoclonal (Biotin) Antibody

Applications:

ELISA, IF, IHC

Format:

Biotin Conjugated

Price:

100 µl/$962

Select

FITC Antibody (APC) LS-C516833

Antibody:

FITC Mouse Monoclonal (APC) Antibody

Applications:

ELISA, IF, IHC

Format:

APC Conjugated

Price:

100 µl/$962

Select

FITC Antibody (PE) LS-C654293

Antibody:

FITC Mouse Monoclonal (PE) Antibody

Applications:

ELISA, IF, IHC

Format:

PE Conjugated

Price:

100 µl/$962

• Unconjugated
• HRP
• Biotin
• APC
• PE

• Unconjugated
• HRP
• Biotin
• More...
  • APC
  • PE

Select

FITC Antibody (Biotin) LS-C689554

Antibody:

FITC Mouse anti-Human Monoclonal (Biotin) Antibody

Reactivity:

All species, Human

Applications:

ICC, IP, WB

Format:

Biotin Conjugated

Price:

100 µl/$319; 200 µl/$365; 500 µl/$520; 1 ml/$598; 2 ml/$828; 5 ml/$1,356

Viewing 1-25 of 107 product results

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