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FITC Antibody (AP) LS‑C66229
FITC antibody LS-C66229 is an AP-conjugated rabbit polyclonal antibody to FITC. Validated for ELISA and WB.
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Species:
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Product Description
FITC antibody LS-C66229 is an AP-conjugated rabbit polyclonal antibody to FITC. Validated for ELISA and WB.
About FITC
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
Specifications
Target
FITC
Host
Rabbit
Clonality
IgG
Polyclonal
Conjugations
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (1:10 - 1:100)
- ELISA (1:100 - 1:500)
- (applications tested for the base form of this product only)
Immunogen
FITC antibody was raised against fluorescein isothiocyanate (FITC).
Specificity
Recognizes fluorescein. No cross-reactivity with immunoglobulins of any species tested. Solid phase hapten-antibody capture.
Usage
Suitable for use in Western Blot and Enzyme Immunoassays. Recommended Dilution: Western Blot: 1:10-1:100 (Recommended substrate: BCIP). ELISA: 1:100-1:500 (Recommended substrate: p-NPP ). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris buffer, pH 8, 10 mg/ml BSA, 0.1% sodium azide, 40% glycerol.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Requested From:
Date Requested: 6/20/2018
Date Requested: 6/20/2018
