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Anti-FITC Antibody (AP) LS-C66229
Most Popular FITC Antibodies
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Details
| FITC | |
| Rabbit | |
| IgG Polyclonal | |
| Alkaline Phosphatase | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- Western blot (1:10 - 1:100)
- ELISA (1:100 - 1:500)
Specificity and Use
| FITC antibody was raised against fluorescein isothiocyanate (FITC). | |
| Recognizes fluorescein. No cross-reactivity with immunoglobulins of any species tested. Solid phase hapten-antibody capture. | |
| Suitable for use in Western Blot and Enzyme Immunoassays. Recommended Dilution: Western Blot: 1:10-1:100 (Recommended substrate: BCIP). ELISA: 1:100-1:500 (Recommended substrate: p-NPP ). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Packaging
| 0.05 M Tris buffer, pH 8, 10 mg/ml BSA, 0.1% sodium azide, 40% glycerol. | |
| Store at 4°C. Do not freeze. | |
| For research use only. |
About FITC
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
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Requested From:
Date Requested: 10/17/2017
Date Requested: 10/17/2017
