Firefly Luciferase antibody LS-C71819 is an unconjugated mouse monoclonal antibody to photinus pyralis Firefly Luciferase. Validated for IF, IHC and WB. Cited in 1 publication.
Photinus pyralis Firefly Luciferase
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Frozen
- Immunofluorescence (10 - 30 µg/ml)
- Western blot (1 - 3 µg/ml)
- (applications tested for the base form of this product only)
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Luciferase protein from Photinus pyralis.
This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.