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Anti-Filamin Antibody (N-Terminus) IHC-plus™ LS-B6314

Note: This antibody replaces LS-C23663


Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Filamin Antibodies

Anti-Filamin Antibody (clone FLMN01, Azide-free) LS-C87615
Mouse Monoclonal [clone FLMN01] (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-Filamin Antibody (N-Terminus) IHC-plus™ LS-B6314
Mouse Monoclonal (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry Image
Anti-Filamin Antibody (clone FLMN01) IHC-plus™ LS-B8516
Mouse Monoclonal [clone FLMN01] (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation


Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
IgG1 Monoclonal


  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation

Specificity and Use

Filamin antibody was raised against human platelet protein.
Recognizes native, full-length human filamin (actin-binding protein) at 270-280kD and 190kD N-terminal calpain cleavage fragment of filamin. Following induction of apoptosis in U937 cells, recognizes 170, 150, and 120kD N-terminal cleavage fragments of the full-length from presumably resulting from cleavage by activated caspase-3. Species cross-reactivity: Chicken, guinea pig, rabbit and rat.
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2000. Immunoprecipitation: 5 ul per 300-500 ul of cell lysate (200-500 ug total protein). Recommended Lysis buffer is PBS, 05% triton X-100 with proteinase inhibitors. Note: For full-length filamin include calpain inhibitors. Incubate for 1 hour at RT or overnight at 4C. For best recovery, use Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody. 4-8% acrylamide gels are recommended for full-length filamin or the 190kD fragment separation and visualization. Immunofluorescence (Frozen): 1:25-1:100. Immunohistochemistry, human-FFPE, 1:50.


0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Filamin


Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6314 dilution 1:50.

Requested From: United States
Date Requested: 10/28/2016

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