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Anti-Filamin Antibody IHC-plus™ LS-B6313

Note: This antibody replaces LS-C23664

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Filamin Antibodies

Anti-Filamin Antibody (clone FLMN01, Azide-free) LS-C87615
Mouse Monoclonal [clone FLMN01] (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-Filamin Antibody (N-Terminus) IHC-plus™ LS-B6314
Mouse Monoclonal (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Filamin Antibody (clone FLMN01) IHC-plus™ LS-B8516
Mouse Monoclonal [clone FLMN01] (IgG1) to Human Filamin
Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human Filamin
Human, Bovine
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human Filamin
Mouse
Human, Bovine (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:250 - 1:1000)
  • Immunoprecipitation

Specificity and Use

Filamin antibody was raised against human platelet protein.
Human filamin (actin-binding protein). Recognizes the 90kD fragment of filamin produced by calpain cleavage. Species cross-reactivity: Human and bovine. Does not react with Mouse or rat.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.

Packaging

No preservatives added.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Filamin


Immunohistochemistry

Immunohistochemistry
Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6313 dilution 1:50.
 

Requested From: United States
Date Requested: 12/7/2016

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