Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG2a) to Human Fibroblasts + Epithelial Cells
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Human Fibroblasts + Epithelial Cells
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Frozen (1:100)
Flow Cytometry (1:50 - 1:200)
Specificity and Use
Human foreskin fibroblasts.
Recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Flow Cytometry: 1:50-1:200; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:100.
PBS, pH 7.4, 0.09% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C.