Human Fibroblasts + Epithelial Cells

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG2a Monoclonal

D7-FIB

Unconjugated. Also available conjugated with RPE.

Protein G purified

Unmodified

Human foreskin fibroblasts.

Recognizes a ~112kD molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies, has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. Is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes. Is routinely tested in flow cytometry on the KG1 cell line.

• IHC - Frozen (1:100)
• Immunoprecipitation
• Flow Cytometry (1:50 - 1:200)

• IHC - Paraffin

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.