Human Fibroblasts

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

TE-7

Unconjugated

Ion exchange chromatography

Unmodified

Whole human thymic stoma cells.

Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

• Immunofluorescence (1:100 - 1:200)
• Flow Cytometry (1:100)

Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Immunofluorescence: 1:100-1:200 (acetone fixed tissue). FACS: 1:100 (fresh or acetone fixed tissue; do not use trypsin to prepare adherent cell lines, use a non-enzymatic detachment procedure.

PBS, 0.09% Sodium Azide, 0.1% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.