Reactivity
Dog
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
FITC,
HRP.
Purification
Purified IgG
Immunogen
Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Dog fibrinogen.
Applications
- Immunofluorescence
- ELISA
- Dot Blot
Usage
The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in dog cells, tissues and body fluids in immunofluorescence and immunoenzyme methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee