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Anti-FHIT Antibody IHC-plus™ LS-B3166

Note: This antibody replaces LS-C6810

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FHIT Antibodies

Anti-FHIT Antibody LS-C50630
Rabbit Polyclonal to Human FHIT
Human
IHC
Unconjugated
Immunohistochemistry Image
Anti-FHIT Antibody (C-Terminus) IHC-plus™ LS-B10125
Rabbit Polyclonal (IgG) to Human FHIT
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human FHIT
Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FHIT
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

FHIT antibody was raised against full length human FHIT fusion protein with GST (glutathione amino transferase).
Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.
Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About FHIT

P49789 NM_002012 NP_002003.1

FHIT Antibody, AP3Aase Antibody, Dinucleosidetriphosphatase Antibody, Fragile histidine triad Antibody, Fragile histidine triad gene Antibody, AP3A hydrolase Antibody, Bis(5-adenosyl)-triphosphatase Antibody, FRA3B Antibody, Tumor suppressor protein Antibody

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways.

Immunohistochemistry

Immunohistochemistry
Anti-FHIT antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3166 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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