Target
Human FGF6
Synonyms
FGF6 | FGF-6 | Fibroblast growth factor 6 | HSTF-2 | HBGF-6 | HST-2 | HST2 | HSTF2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Human, recombinant Fibroblast Growth Factor-6 (FGF-6) from E. coli.
Specificity
Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF-6). Will not neutralize the activity of FGF acidic, FGF basic, rhFGF-4 or rhFGF-5. Based on direct ELISA, this antibody shows ~75% cross-reactivity with rhFGF-5 and less than 10% cross-reactivity with rhFGF-4.
Usage
Suitable for use in Neutralization and ELISA. Neutralization: ND50 ~1-4 ug/ml in the presence of 1 ng/ml of rhFGF-6 and 1 ug/ml of heparin, using the FGF-6 responsive NR6R-3T3 fibroblasts as target cells. ELISA: Using plates coated with 100 ul/well of the capture antibody at 1-2 ug/ml, in combination with 100 ul/well of the detection antibody at 200-400 ng/ml, an ELISA for sample volumes of 100 ul with a range of ~100-6000pg/ml can be obtained.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute with 1 ml sterile PBS.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee