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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGF21 Antibody (clone 2F11) LS‑C133715 Best Seller
FGF21 antibody LS-C133715 is an unconjugated mouse monoclonal antibody to human FGF21. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C133715-100
100 µg (0.5 mg/ml)
$355

Popular FGF21 Products

Anti-FGF21 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5864 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: ELISA
Western blot analysis using anti-FGF-21 (human), mAb (FG204-3) at 1:2000 dilution. 1: Human FGF-21 Fc-protein. 2: Human FGF-21 (FLAG-tagged). 4: Human Jagged-1 Fc-protein (negative control).
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

FGF21 antibody LS-C133715 is an unconjugated mouse monoclonal antibody to human FGF21. Validated for ELISA, IHC and WB.
About FGF21
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Q9NSA1 NM_019113 NP_061986.1

FGF21 Antibody, Fibroblast growth factor 21 Antibody, FGF-21 Antibody


Specifications

Target
Human FGF21
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2F11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FGF21 antibody was raised against fGF21 (AAH18404, 30 a.a. ~ 210 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human FGF21
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Western blot

Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.
Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Western blot

Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.
Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Western blot

Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.
Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to FGF21 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Western blot

Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.
Western blot of FGF21 expression in transfected 293T cell line by FGF21 monoclonal antibody (M01), clone 2F11.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged FGF21 is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 9/23/2018

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