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Anti-FGF2 / Basic FGF Antibody IHC-plus™ LS-B7426

Note: This antibody replaces LS-C104590, LS-C42360

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FGF2 / Basic FGF Antibodies

Anti-FGF2 / Basic FGF Antibody (aa188-210, Magnetic beads) LS-C171562
Rabbit Polyclonal to Human FGF2 / Basic FGF
Human, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image
Anti-FGF2 / Basic FGF Antibody (clone 3D9) IHC-plus™ LS-B10042
Mouse Monoclonal [clone 3D9] (IgG2b) to Human FGF2 / Basic FGF
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human FGF2 / Basic FGF
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human FGF2 / Basic FGF
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA
  • Neutralization (0.25 - 0.4 µg/ml)

Specificity and Use

FGF2 / Basic FGF antibody was raised against e.coli derived recombinant Human FGF-basic.
Human FGF-basic
Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/ml), a concentration of 0.25-0.40 ug/ml of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About FGF2 / Basic FGF

P09038 NM_002006 NP_001997.5

FGF2 Antibody, Basic fibroblast growth factor Antibody, FGFB Antibody, Fibroblast growth factor 2 Antibody, FGF-2 Antibody, Prostatropin Antibody, BFGF Antibody, HBGF-2 Antibody

FGF2 / Basic FGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth.

Immunohistochemistry

Immunohistochemistry
Anti-FGF2 / BFGF antibody IHC of human breast, myoepithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7426 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of FGF2 / BFGF antibody LS-B7426

Western blot

Western blot
Western Blot (reducing) of FGF2 / BFGF antibody LS-B7426

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of FGF2 / BFGF antibody LS-B7426

Requested From: United States
Date Requested: 12/10/2016

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