Target
Human FGF17
Synonyms
FGF17 | Fibroblast growth factor 17 | FGF-13 | FGF-17
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin.
Purification
Immunoaffinity purified
Immunogen
E.coli derived recombinant Human FGF-17.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
Usage
Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-17 (200 ng/ml), a concentration of 2.1-3.2 ug/ml of this antibody is required. ELISA: To detect hFGF-17 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-17 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-17. Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee