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Anti-FEZ1 Antibody (aa278-290) IHC-plus™ LS-B3602

Note: This antibody replaces LS-C54833

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FEZ1 Antibodies

Anti-FEZ1 Antibody (C-Terminus) LS-C34715
Rabbit Polyclonal (IgG) to Human FEZ1
Human, Mouse, Rat
Western blot
Unconjugated
Western blot Image
Anti-FEZ1 Antibody (aa278-290) IHC-plus™ LS-B3602
Goat Polyclonal to Human FEZ1
Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FEZ1 Antibody LS-C310358
Rabbit Polyclonal (IgG) to Human FEZ1
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human FEZ1
Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FEZ1
Goat
Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:128000) & (0.01 - 0.03 µg/ml)
  • ELISA (1:128000)

Specificity and Use

FEZ1 antibody was raised against synthetic peptide C-QNKQKEQRELMKK from an internal region of human FEZ1 (NP_005094.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus (100%); Mouse, Pig (92%); Xenopus, Stickleback, Pufferfish (85%).
LS-E26210 - Lyophilized - 100 µg - $145.00
aa278-290
Human FEZ1. This antibody is expected to recognize isoform 1 (NP_005094.1) only.
Immunohistochemistry: LS-B3602 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3602 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About FEZ1

Q99689 NM_005103 NP_005094.1

FEZ1 Antibody, Zygin-1 Antibody, Zygin I Antibody

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Immunohistochemistry

Immunohistochemistry
Anti-FEZ1 antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3602 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FEZ1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3602 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Human Brain (frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/10/2016

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