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Anti-FEM1B Antibody (aa609-622) IHC-plus™ LS-B568

Note: This antibody replaces LS-C379, LS-C65820

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FEM1B Antibodies

Anti-FEM1B Antibody (aa609-622) IHC-plus™ LS-B568
Rabbit Polyclonal to Human FEM1B
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FEM1B Antibody LS-C128907
Goat Polyclonal (IgG) to Human FEM1B
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human FEM1B
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Human FEM1B
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 1 µg/ml)

Specificity and Use

FEM1B antibody was raised against synthetic peptide from human FEM1B. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%); Opossum (86%).
LS-E29935 - Liquid - 50 µg - $145.00
aa609-622
Peptide (DINYQDQIPRTLEE) corresponding to amino acids 609 to 622 of human F1Aa This sequence is identical to the corresponding sequence of mouse FEM1b.
Immunohistochemistry: LS-B568 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B568 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About FEM1B

Q9UK73 NM_015322 NP_056137.1

FEM1B Antibody, F1AA Antibody, FEM-1 (C.elegans) homolog b Antibody, FEM1-beta Antibody, FIAA Antibody, KIAA0396 Antibody, F1A-ALPHA Antibody, Fem-1 homolog b (C. elegans) Antibody, Protein fem-1 homolog B Antibody

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aa and FEM1b (1,2). F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aa is cleaved by CED-3 and caspase (3). FEM-1/F1Aa associates with CED-4 and its mammalian homologue Apaf-1 (3). Overexpression of F1Aa induces apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-FEM1B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B568 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 ug/ml.

Western blot

Western blot
Western blot of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 ug/ml.
 

Requested From: United States
Date Requested: 12/6/2016

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