Target
Human FCN2 / Ficolin-2
Synonyms
FCN2 | 37 kDa elastin-binding protein | EBP-37 | FCNL | Ficolin B | Ficolin-beta | Hucolin | Ficolin-2 | L-ficolin | p35 | Serum lectin p35 | Ficolin-B
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhFicolin-2.
Specificity
Recognizes human Ficolin-2. Does not cross-react with rhFicolin-3.
Usage
Western Blot, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Phosphate Buffered Saline (PBS)
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee