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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FCGR2 / CD32 Antibody (phospho‑Tyr292) LS‑C198761
CD32 antibody LS-C198761 is an unconjugated rabbit polyclonal antibody to CD32 (FCGR2) from human, mouse and rat. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C198761-50
50 µl
Unavailable
LS-C198761-100
100 µl
Unavailable

Popular FCGR2 / CD32 Products

Staining of lysed whole blood with FITC anti-human CD19 (HIB19) and 0.25 ug of APC Mouse IgG1, K isotype control (left) or APC anti-human CD32 (6C4) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Flow cytometry of FCGR2 / CD32 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, Functional Assay
Flow cytometry of FCGR2 / CD32 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, Functional Assay
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry

Product Description

CD32 antibody LS-C198761 is an unconjugated rabbit polyclonal antibody to CD32 (FCGR2) from human, mouse and rat. Validated for Peptide-ELISA and WB.
About FCGR2 / CD32
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Monoclonal antibodies can distinguish between CD32A and CD32B.

Specifications

Target
Human FCGR2 / CD32
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Immunogen
FCGR2 / CD32 antibody was raised against synthetic peptide derived from human CD32 around the phosphorylation site of Tyr292.
Epitope
pTyr292
Specificity
CD32 (Phospho-Tyr292) Antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine292.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/22/2018

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