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Anti-FCGR2 / CD32 Antibody (clone 16D6) LS-C114762

Catalog Size Price
LS-C114762-100 100 µl (0.49 mg/ml) Unavailable

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Staining of lysed whole blood with FITC anti-human CD19 (HIB19) and 0.25 ug of APC Mouse IgG1, K isotype control (left) or APC anti-human CD32 (6C4) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
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Flow cytometry of FCGR2 / CD32 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Flow cytometry of FCGR2 / CD32 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
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100% Guaranteed
Mouse Monoclonal [clone 16D6] (IgG1) to Human FCGR2 / CD32
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human FCGR2 / CD32
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [16D6]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)

Specificity and Use

FCGR2 / CD32 antibody was raised against full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Reacts with CD32A, not yet tested for cross reactivity with CD32B.
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About FCGR2 / CD32

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Monoclonal antibodies can distinguish between CD32A and CD32B.

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded bladder tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded bladder tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-FCGR2A mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
Anti-FCGR2A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FCGR2A antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FCGR2A antibody, and then analyzed by flow cytometry.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY FCGR2A (Red) or empty vector control plasmid (Blue) were immunostained with anti-FCGR2A mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY FCGR2A (Red) or empty vector control plasmid (Blue) were immunostained with anti-FCGR2A mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

Requested From: 
Date Requested: 5/24/2018

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