Human FceR1 / Fc Epsilon Receptor 1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

9E1

Unconjugated. Also available conjugated with RPE.

Affinity purified

Unmodified

Recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two disulfide linked gamma subunits. Clone Recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence
• Western blot
• Flow Cytometry (1:25 - 1:50)
• ELISA

Immunohistochemistry: LS-B3150 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3150 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

PBS, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.