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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FBXW10 Antibody (aa180‑229) LS‑C81722
FBXW10 antibody LS-C81722 is an unconjugated rabbit polyclonal antibody to FBXW10 from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C81722-50
50 µg
Unavailable

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Product Description

FBXW10 antibody LS-C81722 is an unconjugated rabbit polyclonal antibody to FBXW10 from human and monkey. Validated for WB.
About FBXW10
Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains. Q5XX13 NM_031456 NP_113644.3

FBXW10 Antibody, Fbw10 Antibody, HREP Antibody, SM25H2 Antibody, C17ORF1 Antibody, C17ORF1A Antibody, SM2SH2 Antibody


Specifications

Target
Human FBXW10
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
FBXW10 antibody was raised against synthetic peptide located between aa180-229 of human FBXW10 (Q5XX13). Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%); Gibbon, Monkey (85%).
Blocking Peptide
LS-E18851 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81722. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human FBXW10
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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