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Anti-FBXO4 / FBX4 Antibody (N-Terminus) LS-C18995


Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Anti-FBXO4 / FBX4 Antibody (aa231-260, Biotin) LS-C257123
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human FBXO4 / FBX4
Human, Mouse
ICC, Immunofluorescence, Western blot


Human FBXO4 / FBX4
Human, Mouse (tested or 100% immunogen sequence identity)


  • ICC
  • Immunofluorescence
  • Western blot

Specificity and Use

FBXO4 / FBX4 antibody was raised against synthetic peptide from human FBXO4.
Synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).
This antiserum has been tested for use in western blotting and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kD in size by western blotting in the appropriate cell lysate or extract. Western Blot 1:500 - 1:3000; IMMUNOCYTOCHEMISTRY 1:100 - 1:500.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About FBXO4 / FBX4

Q9UKT5 NM_012176 Q9UKT5

FBXO4 Antibody, F-box protein 4 Antibody, F-box protein Fbx4 Antibody, FBX4 Antibody, F-box only protein 4 Antibody

FBXO4 / FBX4 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.


Immunocytochemistry staining of NIH-3T3 cells using anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution.

Western blot

Western blot
Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 antibody was used at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG.

Requested From: United States
Date Requested: 10/25/2016

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