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Anti-FANCG Antibody (C-Terminus) IHC-plus™ LS-B2997

Note: This antibody replaces LS-C54813

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

FANCG Antibody LS-B2997 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-FANCG antibody IHC of human uterus.
Immunohistochemistry
Anti-FANCG antibody IHC of human spleen.
Western blot
Antibody (0.5 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer).

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Goat Polyclonal to Human FANCG
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FANCG
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (3 µg/ml), Western blot (0.5 - 1 µg/ml), ELISA (1:32000)

Specificity and Use

FANCG antibody was raised against synthetic peptide LEEFRTSLPKSCDL from the C-terminus of human FANCG (NP_004620.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Dog (86%).
LS-E25606 - Lyophilized - 100 µg - $145.00
C-Terminus
Human FANCG.
Immunohistochemistry: LS-B2997 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2997 was determined to be 3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About FANCG

O15287 NM_004629 NP_004620.1

FANCG Antibody, DNA repair protein XRCC9 Antibody, Fanconi anemia group G protein Antibody, FAG Antibody, Protein FACG Antibody, XRCC9 Antibody

FANCG, involved in Fanconi anemia, confers resistance to both hygromycin and mitomycin C. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies. A minimum of 8 FA genes have been identified.

Immunohistochemistry

Immunohistochemistry
Anti-FANCG antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2997 concentration 3 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FANCG antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2997 concentration 3 ug/ml.

Western blot

Western blot
Antibody (0.5 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 6/2/2015

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