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Anti-FANCG Antibody (aa1-12) IHC-plus™ LS-B368

Note: This antibody replaces LS-C18975, LS-C56852

Ordering

Wt. Vol. Conc. Price
50 µg - 0.93 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FANCG Antibodies

Anti-FANCG Antibody (aa1-12) IHC-plus™ LS-B368
Rabbit Polyclonal to Human FANCG
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FANCG Antibody (aa609-622) IHC-plus™ LS-B2997
Goat Polyclonal to Human FANCG
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human FANCG
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human FANCG
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

FANCG antibody was raised against synthetic peptide from human FANCG.
aa1-12
Amino acids 1-12 of human FANCG protein.
Immunohistochemistry: LS-B368 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B368 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About FANCG

O15287 NM_004629 NP_004620.1

FANCG Antibody, DNA repair protein XRCC9 Antibody, Fanconi anemia group G protein Antibody, FAG Antibody, Protein FACG Antibody, XRCC9 Antibody

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair.

Immunohistochemistry

Immunohistochemistry
Anti-FANCG antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B368 concentration 5 ug/ml.

Western blot

Western blot
Anti-FANCG antibody - Western Blot. Western blot of affinity purified anti-FANCG antibody shows detection of a band at ~69 kD (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/8/2016

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