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Anti-FANCF Antibody IHC-plus™ LS-B210

Note: This antibody replaces LS-C19024
Catalog Size Price
LS-B210-50 50 µg (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human FANCF
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot
  • ELISA (1:15000 - 1:60000)

Specificity and Use

FANCF antibody was raised against synthetic peptide from human FANCF.
An internal amino acid sequence of human FANCF.
Immunohistochemistry: LS-B210 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B210 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.


Q9NPI8 NM_022725 NP_073562.1

FANCF Antibody, Fanconi anemia group F protein Antibody, Protein FACF Antibody, FAF Antibody

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair.


Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B210 concentration 5 ug/ml.

Western blot

Western blot
Anti-FANCF Antibody - Western Blot. Western blot of affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kD in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kD appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.

Requested From: 
Date Requested: 3/23/2018

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