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Anti-FANCF Antibody IHC-plus™ LS-B210

Note: This antibody replaces LS-C19024
Catalog Size Price
LS-B210-50 50 µg (1 mg/ml) Unavailable

Related Products

6 FANCF Antibodies

Most Popular FANCF Antibodies

Anti-FANCF Antibody IHC-plus™ LS-B210
Rabbit Polyclonal to Human FANCF
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FANCF Antibody (clone 3C4) LS-C197193
Mouse Monoclonal [clone 3C4] (IgG1,k) to Human FANCF
Human
Immunofluorescence, ELISA
Unconjugated
Immunofluorescence Image
Anti-FANCF Antibody LS-C400743
Rabbit Polyclonal (IgG) to Human FANCF
Human
IHC, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FANCF Antibody LS-C401662
Rabbit Polyclonal (IgG) to Human FANCF
Human
IHC, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FANCF Antibody LS-C404961
Rabbit Polyclonal (IgG) to Human FANCF
Human
IHC, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human FANCF
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human FANCF
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot
  • ELISA (1:15000 - 1:60000)

Specificity and Use

FANCF antibody was raised against synthetic peptide from human FANCF.
An internal amino acid sequence of human FANCF.
Immunohistochemistry: LS-B210 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B210 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About FANCF

Q9NPI8 NM_022725 NP_073562.1

FANCF Antibody, Fanconi anemia group F protein Antibody, Protein FACF Antibody, FAF Antibody

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair.

Immunohistochemistry

Immunohistochemistry
Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B210 concentration 5 ug/ml.

Western blot

Western blot
Anti-FANCF Antibody - Western Blot. Western blot of affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kD in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kD appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.

Requested From: 
Date Requested: 10/16/2017

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